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Figure 2.
Figure 2. The extra density found near active site of PfGAPDH.
The stereo view of the additional electron density shows the
excellent correspondence between the difference density (green,
contour level +3 sigma) prior to refinement with the AEBSF and
the Sigma-A weighted electron density after placement and
refinement with AEBSF (blue, contour level +1 sigma). PfGAPDH,
the associated NAD^+, and the AEBSF are shown with carbon in
gold, oxygen in red, nitrogen in blue, phosphorous in magenta,
and sulfur in green. A superimposed region of the human GAPDH
structure (PDB: 1ZNQ, shown in gray) shows the structural
similarity of the active site, with the exception of a threonine
found at the site corresponding to PfGAPDH Asn185. Hydrogen
bonds or other favorable electronstatic interactions between the
PfGAPDH and the AEBSF, or the NAD^+ and the AEBSF are shown in
dashed lines.
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