Figure 2.
Figure 2. Model of L30e in its ribosomal environment. (a)
Wheat germ L30e amino acid sequence with cylinders and arrows
indicating -helical
and -strand
secondary structure, respectively. (b) Top, L30e density at
different contour levels (2.5 and 1 ).
Bottom, adjusted homology model of L30e in transparent EM
density and L30e crystal structure (green, PDB entry, chain
D1NMUD). Note the different conformation of 4.
(c) Ribosomal environment of L30e (accession code 1YSH in the
Protein Data Bank). Blue, 25S RNA; orange, L37Ae and unknown
protein cluster II; magenta, L30e; yellow, 18S RNA; green, S13e
(S15p). Ribosome indicates orientation. (d) L30e conformation in
the post state and in the eEF2-bound state of the 80S ribosome.
The same region of the 60S subunit is shown in the post state
(left, wg ribosome, filtered to 13) and in the eEF2-bound state
(right, yeast ribosome at 12). B4 and eB9 indicate intersubunit
bridges. Note the differences in the density for 4
of L30e (magenta).
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2005,
12,
467-468)
copyright 2005.
-helical
and 
-strand
secondary structure, respectively. (b) Top, L30e density at
different contour levels (2.5 and 1 
).
Bottom, adjusted homology model of L30e in transparent EM
density and L30e crystal structure (green, PDB entry, chain
D1NMUD). Note the different conformation of