Figure 2.
Figure 2 Three-dimensional structure of T. maritima A
SR4. (A) Superposition of the NMR structure family. Chemical
shifts for residues 379 -383 (the loop between S4 and S5) were
not identified and this segment is relatively disordered in the
family. (B) Helix arrangement in TmSR4. Helix S1 (residues 322
-329), helix S2 (residues 334 -342), helix S3 (residues 353
-361), helix S4 (residues 364 -377) and helix S5 (residues 385
-393) were identified from secondary 13C[ /
]shifts,
d[NN] (i, i+1) NOEs and 3J[NH ]coupling
constants. (C) Hydrophobic core of TmSR4 formed from nonpolar
residues found in all the five helices. (D) Superposition of
TmSR4 (blue), the T. aquaticus A
SR4 in its DNA-bound conformation (red) (Campbell et al, 2002)
and the T. thermophilus A
SR4 in its holoenzyme conformation (green) (Vassylyev et al,
2002).
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO J
(2004,
23,
2952-2962)
copyright 2004.
A
SR4. (A) Superposition of the NMR structure family. Chemical
shifts for residues 379 -383 (the loop between S4 and S5) were
not identified and this segment is relatively disordered in the
family. (B) Helix arrangement in TmSR4. Helix S1 (residues 322
-329), helix S2 (residues 334 -342), helix S3 (residues 353
-361), helix S4 (residues 364 -377) and helix S5 (residues 385
-393) were identified from secondary 13C[ 
/
]shifts,
d[NN] (i, i+1) NOEs and 3J[NH