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Figure 2.
Figure 2. PON1's active site viewed from above the propeller.
(a) Central tunnel of the propeller with the two calcium atoms,
and the side chains of the residues found to be mutated in the
newly evolved PON1 variants for esterase and lactonase (orange)
or for phosphotriesterase activity (yellow), including the R192Q
human polymorphism (in the rePON1-G2E6 variant, this position is
a lysine). The putative catalytic His-His dyad is red (see text
and Fig. 4). (b) A surface view of the active site. Lys70, Tyr71
and Phe347 are shown as sticks to permit a better view of the
active site. At the deepest point of the cavity lies the upper
calcium atom (Ca1, green) to which a phosphate ion is bound.
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