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Figure 2.
Figure 2. Structure-Based Alignment of Human Apyrase with
Other Apyrase ProteinsThe sequence of human apyrase (residues
Pro1–Ile333) is shown with the elements of secondary structure
indicated above the alignment. Gray arrows and cylinders mark β
strands and α helices, respectively, with crosses denoting
residues that are disordered in the present apo and substrate
analog structures. The sequences of human, rat, bed bug, and
sand fly (Phlebotomus) are shown; in these cases the enzymatic
activity has been established by biochemical analysis. Residues
that are important for nucleotide and Ca^2+ binding are
indicated by pluses and asterisks, respectively. Chemically
similar residues in the apyrase family are colored red and
residues conserved in the vertebrate and insect subfamilies in
blue and green, respectively. We refer to the human apyrase
structure using the numbering system of the mature protein.
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