Figure 2.
Fig. 2. Structural comparison of the 18HA0 cleavage site with
other HAs. HA2 domains for human H3 HA0 (PDB ID code: 1ha0 [PDB]
) and cleaved avian H5 HA1/HA2 (PDB ID code: 1jsm [PDB]
) (50) were aligned with 18HA0. The cleavage sites are colored
(A) green for human H3 HA0, (B) red for 18HA0 and (C) orange for
H5 HA1/HA2. RA329Q, ArgA329 GlnA329. (D)
Overlay of cleavage loops of H3 HA0, H1 HA0, and avian H5
HA1/HA2. The two views differ by a rotation of 90° about the
threefold vertical axis. (E) Surface views showing the trimer
interface and the position of the cleavage loop. (F) Removal of
the cleavage loop reveals the electronegative cavity that it
masks. Arg329 is colored blue and N-acetyl-glucosamines,
indicating the nearby glycosylation sites, are colored gold. (A)
to (D) were generated as in Fig. 1, and (E) and (F) were
generated with MSMS (46) through the program VMD (47).
The above figure is reprinted
by permission from the AAAs:
Science
(2004,
303,
1866-1870)
copyright 2004.
GlnA329. (D)
Overlay of cleavage loops of H3 HA0, H1 HA0, and avian H5
HA1/HA2. The two views differ by a rotation of 90° about the
threefold vertical axis. (E) Surface views showing the trimer
interface and the position of the cleavage loop. (F) Removal of
the cleavage loop reveals the electronegative cavity that it
masks. Arg329 is colored blue and N-acetyl-glucosamines,
indicating the nearby glycosylation sites, are colored gold. (A)
to (D) were generated as in Fig. 1, and (E) and (F) were
generated with MSMS (46) through the program VMD (47).