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Figure 2.
Figure 2. Interaction between the mDvl1 PDZ Domain and
Fz7(A) ^15N-HSQC spectra of free Fz7 peptide and Fz7 peptide
bound to the PDZ domain of mDvl1. The red contour lines
represent spectra of the free form of the PDZ domain when no Fz7
peptide (GKTLQSWRRFYH) was present, the green lines (upper
inset) represent spectra of partially bound forms of the PDZ
domain when 0.86 mM Fz7 peptide was present, and the blue lines
represent spectra of the fully bound forms of the PDZ domain
when 10 mM Fz7 peptide was present. The concentration of the PDZ
domain was 1.1 mM. The two insets show the enlarged regions
where the chemical-shift perturbations were small (lower inset)
and large (upper inset). In the upper inset, the signals from
the same residue but in different spectra were placed in smaller
boxes.(B) The figure shows the worm representation of the
backbone structure of the mDvl1 PDZ domain. The thickness of the
worm is proportional to the weighted sum (in Hz) of the ^1H and
^15N shifts upon binding by the Fz7 peptide (see A), and the
increasing chemical-shift perturbation is shown (blue, low; red,
high).(C) Ribbon diagram of the PDZ domain structure. The
binding site of the Fz7 peptide identified from the
chemical-shift perturbation studies is indicated.
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