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Figure 2.
Figure 2. Trisoxazole-containing macrolide toxins bind to the
same site on actin as gelsolin domain 1. (a) The structure of
the actin -jaspisamide A complex in two orientations where actin
is depicted in a ribbon representation and the toxin is shown as
a space-filling model in red. Actin subdomains 1 -4 are labeled.
(b) A space-filling representation of the residues on actin that
interact with kabiramide C (left) and gelsolin domain 1 (right).
The coordinates for gelsolin domain 1 were obtained from the
RCSB (PDB entry 1EQY)5, 6. (c) Overlay of kabiramide C (red,
space-filling representation) and gelsolin domain 1 (blue) based
on the superposition of the actin in their respective complexes.
For gelsolin domain 1 the actin-binding helix, Ser70 -Leu88, is
shown in space-filling representation. (d) Kabiramide C binding
site on actin. Toxin is shown as ball and stick representation
in cyan; labeled amino acid residues contacting kabiramide C are
shown in CPK colors as a space-filling model.
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