Figure 2.
FIG. 2. Fluorescence titration of UP1 with TR2-6F ( o ) and
TR2-11F ( . Fluorescent signal of
6-MI is strongly influenced by its environment. In TR2-6F, 6-MI
becomes fully unstacked and solvent-exposed upon UP1 binding. In
TR2-11F, 6-MI stacks with neighboring Gua-10 when bound to UP1.
Excitation and emission wavelengths were 340 and 430 nm,
respectively, and both titrations were performed in 20 mM MES,
pH 6.0, and 100 mM NaCl at 25 °C. Total gain-of-signal for
TR2-6F was 3.3x the base-line; for TR2-11F, it was 1.2x
base-line.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2003,
278,
42300-42306)
copyright 2003.
. Fluorescent signal of
6-MI is strongly influenced by its environment. In TR2-6F, 6-MI
becomes fully unstacked and solvent-exposed upon UP1 binding. In
TR2-11F, 6-MI stacks with neighboring Gua-10 when bound to UP1.
Excitation and emission wavelengths were 340 and 430 nm,
respectively, and both titrations were performed in 20 mM MES,
pH 6.0, and 100 mM NaCl at 25 °C. Total gain-of-signal for
TR2-6F was 3.3x the base-line; for TR2-11F, it was 1.2x
base-line.