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Figure 2.
Figure 2. Electron Density of the Editing Substrates(A)
Simulated omit map (Brunger et al., 1998) for the pretransfer
substrate analog (NvaAMS) in the editing (top) and synthetic
(bottom) active site. Resolution is 2.2 Å. In both
molecules, the ribose is in the C2′ endo conformation.(B)
Location of the NvaAMS in the synthetic and editing active sites
of LeuRSTT.(C) Unbiased difference map (2.0 Å resolution)
for the posttransfer editing substrate analog (Nva2AA) in the
editing site. The ribose is in the C3′ endo conformation.(D)
Competitive inhibition of E. coli LeuRS editing of Ile-tRNA^Leu
by Nva2AA. Editing of Ile-tRNA^Leu by wild-type E. coli LeuRS in
the absence of inhibitor exhibited a K[M] of 0.2 μM.
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