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Figure 2.
Fig. 2. (A) Scheme showing how the different structures in Fig.
1 can be arranged to follow the dihydroxylation reaction
catalyzed by NDO. Naphthalene and indole are both substrates for
the enzyme, and we have used them interchangeably in different
studies of the enzyme (23). For simplicity we show only
naphthalene here. The structures are as follows: 1, the resting
enzyme with oxidized Rieske center and ferrous active site
[Protein Data Bank (PDB) code 1O7H]; 2, the reduced enzyme (PDB
code 1O7W); 3, binary dioxgen complex (PDB code 1O7M); 4, binary
substrate complex, structures with both indole and naphthalene
[PDB codes 1EG9 (indole) and 1O7G (naphthalene)]; 5, ternary
substrate dioxygen species, structure with indole (PDB code
1O7N); and 6, product naphthalene cis-1,2-dihydrodiol (PDB code
1O7P). [2Fe-2S] refers to the nearest Rieske iron-sulfur
cluster. (B) Chemical steps in the dioxygenation reaction
carried out by Rieske dioxygenases.
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