Figure 4 - full size

 

Figure 4.
Figure 4. Lactose Binding to TcTS(A) Complete BIAcore sensorgrams showing the interaction between the inactive mutant Asp59Asn and sialic acid (continuous line). Lactose (10 mM) was injected after equilibrating the protein in the presence (continuous line) or absence (dashed line) of sialic acid.(b) Detailed view of the interaction between TcTS and lactose, demonstrating that the acceptor substrate only binds to TcTS when sialic acid is present.

The above figure is reprinted by permission from Cell Press: Mol Cell (2002, 10, 757-768) copyright 2002.