Figure 2 - full size

 

Figure 2.
Figure 2 Active-site density of BCAT inhibited by Tris. The map was calculated from a structure that does not contain Tris. This is the first example in the Protein Data Bank where a buffer has formed a covalent intermediate with an enzyme active site.

The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2001, 57, 506-515) copyright 2001.