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Figure 2.
Figure 2. Stereo drawings to show isopropanol binding reducing
the disorder in the interior of TLN. A shows the refined TLN-0
structure. B and C show the refined TLN-10 structure. Red cpk
spheres denote bound water molecules in A, B and C. A.
2mF[o(nat)]-DF[c] map (contoured at 1.2  ),
at a resolution of 1.65 Å. The side chains of M120 and
E143 are disordered. B. 2mF[o(ipa,10)]-DF[c] map (contoured at
1.2 ),
at a resolution of 1.95 Å. The side chains of M120 and
E143 are in a single conformer, and L144 has changed
conformation, highlighting a  concerted
re-packing  .[11]
C. mF[o(ipa,10)]-mF[o(nat)] difference density map (contoured at
±5.0 )
(1.95 Å). Positive and negative difference density are
shown in green and red, respectively. For clarity, the alternate
B conformers of the M120 and E143 side chains, and the original
conformation of L144 in the TLN-0 structure have been
superimposed (colored yellow). The phases were calculated from
the refined TLN-0 model (see Materials and Methods).
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