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Figure 1.
Fig. 1. Antigenic activity of the parent and
retro-inverso (RI) peptide in TFE measured using the biosensor
instrument BIAcore. The successive steps of the assay and the
controls are described under "Materials and Methods." The
results reflect the effect of TFE on the antigenicity of
peptides. The antibodies used as probes were not in contact with
the TFE solution during the test. The peptides tested are
described in the figure. The antibodies tested were from guinea
pig antisera (diluted 1:50) raised against the L-peptide 141-159
(a) and RI-peptide 141-159 (b) and from rabbit antisera (diluted
1:20) raised against the L-peptide 141-159 (c) and RI-peptide
141-159 (d). The mouse monoclonal antibody 4x11 directed against
the parent peptide IRGERA was tested as a control with the L-
and retro-inverso peptides IRGERA (e). The results are expressed
in RU.
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