Figure 1.
Figure 1. Model of the vert,
similar 590 kDa complex observed in solution studies of the NC10
scFv(5)-NA, in which the scFv(5) is dimeric and bivalent [Kortt
et al 1997]. Model is shown as a bold C^αtrace. The NA
tetramers (green and purple) are crosslinked by scFv(5)
molecules. The Fv domain bound to the green NA is shown with
V[L]yellow and V[H]orange, while the Fv domain bound to the
purple NA is shown with V[L]light blue and V[H]dark blue. The
scFv(5) is thus shown with one V[L]yellow and the other light
blue, and one V[H]orange and the other dark-blue. The complex
has point group 422 symmetry. Drawn with MOLSCRIPT [Kraulis
1991] and RASTER3D [Bacon and Anderson 1989 and Merritt and
Murphy 1994]. (a) View with the molecular 4-fold axis vertical.
(b) View with the molecular 4-fold axis out of the page.
The above figure is reprinted
by permission from Elsevier:
J Mol Biol
(1998,
279,
901-910)
copyright 1998.
vert,
similar 590 kDa complex observed in solution studies of the NC10
scFv(5)-NA, in which the scFv(5) is dimeric and bivalent [Kortt
et al 1997]. Model is shown as a bold C^αtrace. The NA
tetramers (green and purple) are crosslinked by scFv(5)
molecules. The Fv domain bound to the green NA is shown with
V[L]yellow and V[H]orange, while the Fv domain bound to the
purple NA is shown with V[L]light blue and V[H]dark blue. The
scFv(5) is thus shown with one V[L]yellow and the other light
blue, and one V[H]orange and the other dark-blue. The complex
has point group 422 symmetry. Drawn with MOLSCRIPT [Kraulis
1991] and RASTER3D [Bacon and Anderson 1989 and Merritt and
Murphy 1994]. (a) View with the molecular 4-fold axis vertical.
(b) View with the molecular 4-fold axis out of the page.