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Figure 2.
Figure 2. A stereo view of the difference Fourier maps at 1.8 Å showing the difference in electron density between
mutants and wild-type gene V protein. Amplitudes (Fmut - FWT ) were used, where Fmut and FWT are the observed
structure amplitudes for the mutant and wild-type structure. The phases were calculated from the refined gene V protein
model. The V35I and I47V maps are contoured at 3s and the V35I/I47V map at 2.5s for positive and negative contours,
where s is the root-mean-square density throughout the cell. Positive and negative electronic densities are displayed
in green and red, respectively. Yellow is red and green on top of each other. Coordinates are those for the wild-type
gene V protein. Nomenclature for atoms is as follows: CD1 and CD2 are equivalent to C
d1
and C
d2
, CG1 and CG2 are
equivalent to C
d1
and C
d2
, and CB is equivalent to C
b
. (a) V35I; (b) I47V; (c) V35I/I47V.
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