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Figure 1.
Fig. 1. Ca region f a'3C-constanttimeHSQCcorrelationexperi-
ment.Peaksare labeled by the single-letter codeandsequencenumber,
withthe Ca peaksdistinguishedfrom C@ andC6peaks by an enclos-
ing box.Theconstanttimeperiod was 17 ms,withatotal of 189 incre-
ments,eachasum of 64 transients.Processing was performedwith
TRIADsoftware(Tripos Associates). Theintensityofthe first point for
achID was halved, followed by apodization with asquared sine wave,
shifted by 80". Each FID was zero-filled to 2,048points,Fouriertrans-
formed,phased,andthen bascline correted with apolynomialfit.Pairs
ofinterferogramsfrom 512 incrementswereshuffled to separaereal
ndmaginaryparts.Thesecondtransform was perforedftersimi-
lar halvingofthefirstpointandapodization,followed by zerofilling
o 1,024 oints.Thespectrum was referenced to theTSP peak t 0 ppm
forbothcarbonandproton dimensions; two wl sweepwidths were added
to te ormer to obtainthewaleforthisregion.
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