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Figure 1.
Overall structures of heparinase I. A, degradation of heparin
and heparin sulfate by heparinases I–III. The naming of the
sugar sites follows the nomenclature introduced by Davies et al.
(26). The arrows indicate the cleavage site. B, schematic
representation of the heparinase I-H151A-heparin oligosaccharide
(HE[12]) complex. The heparin is shown in stick representation,
and Ca^2+ is shown as an orange sphere. The thumb domain is
colored blue, and the tip of the thumb (indicated by the blue
arrow) is colored cyan. C, close-up of the thumb domain rainbow
(blue, N terminus; red, C terminus). D, bipyramidal coordination
of a Ca^2+ ion in apo heparinase I structure. These and
subsequent figures were prepared with PyMOL (27).
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