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Figure 1.
(a–c) Various lengths of N-terminal Cys-(CH[2])[6]–fused
Thr78 peptides were cross-linked to beads (a) and then tested
for their ability to precipitate PLK1 from mitotic HeLa lysates.
The phosphorylated Thr78 residue ('T' in red) and the invariable
Ser77 residue ('S' in blue) crucial for PBD binding are
indicated in a (above right). Immunoblots with antibody to PLK1
show levels of PLK1 coprecipitated with the indicated peptides.
A shortened form of the synthetic peptide optimized for PLK1 PBD
binding (MQSpTPL)^13 was included for comparison. Numbers
indicate efficiency of PLK1 precipitation by each peptide
relative to the PLK1 signal in the input. (d) A 6-mer Thr78
peptide (LHSpTAI) analogous to the synthetic optimal peptide
(MQSpTPL) was tested for PLK1 binding as in a–c.
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