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Figure 1.
Structure of human DDX19. A, overview of DDX19 with ADP bound
and the N-terminal flanking helix in the central cleft. The
Arg^429 side chain that acts as an arginine finger is presented
as sticks. B, schematic representation of the cleft-inserted
helix with the two conserved domains of the protein, shown in
the same view as in panel A. Residues that are conserved in
DDX25 are shown in blue. C, overview of the DDX19-RNA complex,
with Mg-ADPNP bound in the central cleft. The Arg^429 side chain
is presented as sticks. D, detail of the RNA binding site of the
DDX19-RNA complex. E, detail of the nucleotide binding site in
the open conformation, with the electron density (2F[obs] –
F[calc]) for ADP rendered at 1.5 σ. F, detail of the nucleotide
binding site in the RNA complex, with the electron density
(2F[obs] – F[calc]) for Mg-ADPNP rendered at 1.5 σ. In all
panels, the conserved domain-1 (yellow), the conserved domain-2
(red), and the N-terminal flanking sequence (green) are
indicated.
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