|
Figure 1.
Figure 1. Binding Kinetic Sensorgrams for the IL-7/IL-7Rα
Interaction Determined Using SPR
(A) Binding kinetics
between unglycosylated IL-7 and IL-7Rα, both from E. coli (EC).
(B and C) Binding kinetics of IL-7 (EC) to glycosylated
IL-7Rα from Chinese hamster ovary (CHO) and Schneider (S2)
insect cells, respectively.
(D) Binding kinetics of IL-7 to
PNGase F-treated IL-7Rα (S2).
(E) Binding kinetics between
IL-7 (EC) and Endo H-treated IL-7Rα (S2).
The binding
kinetics sensorgrams are in black. The red curves are global
fits of the SPR data to a two-step binding reaction model to
determine the binding kinetics.
|
