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Figure 1.
(a) Location of H3K79 and H4K20 (red) on the unmodified NCP
structure (surface representation; PDB 1AOI). Histones H2A, H2B,
H3 and H4 are shown in light yellow, red, blue and green,
respectively. (b) H3K[c]79me2 adopts an alternative side chain
conformation. The structures of the NCP containing H3[c]79me2
(light blue and light green) were superimposed onto unmodified
NCP (dark blue and dark green). H3K[c]79me2 is shown in yellow
and unmodified K79 is shown in red. (c) Electrostatic potential
of the same region in NCP containing H3K[c]79me2 as shown in b.
(d) The equivalent region for unmodified NCP. Red indicates
negative surface potential and blue indicates positive surface
potential, scaled from -15 to +15. Note the small hydrophobic
cavity (indicated with an arrow in c) that is uncovered by the
reorientation of H3K[c]79me2. (e) Superposition of nucleosomes
with H4K[c]20me3 (light green, light blue) with unmodified NCP
(dark green, dark blue). (f) Superposition of H4 tails from
published nucleosome structures. Blue, human NCP (PDB 2CV5)^19;
yellow, X. laevis NCP (PDB 1KX5)^20; red, acetylated histone H4
(PDB 16EI)^21; green, NCP with H4K[c]20me3 (this work, PDB 3C1B).
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