|
Figure 1.
Fig. 1. Cross-stand ladders in self-assembled β-sheets and
the PSAM system. (a) Schematic drawings showing how the sequence
and register of a self-assembling peptide determine ladder
composition. A simplified β-sheet is shown as a connection of
α-carbons, with side-chain spheres colored to indicate chemical
identity. Only an antiparallel assembly is shown. (b) Scheme
illustrating the PSAM concept and ladder mutations: a segment of
peptide self-assembly is excised, linked, and capped. Mutations,
shown as green spheres, can then be introduced into ladder
positions within the PSAM scaffold. (c) Cartoon representations
of the PSAM scaffolds used (left: large scaffold, i.e., OspA +
3bh; right: small scaffold, i.e., natural OspA). The SLB in each
scaffold is colored blue with the α-carbons of mutated ladder
positions shown as spheres. The side chains of these ladders are
all located on the same face of the β-sheet.
|
