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Figure 1.
Fig. 1. The FNfn10 scaffold and loop sequences of MBP-binding
monobodies. (a) A schematic of the FNIII scaffold. The seven
β-strands are labeled A–G and three loops used for interface
design (BC, DE and FG) are labeled. (b) The amino acid sequences
of the loop regions of YS-only monobody YS1 and monobodies from
the YSX library. For the latter group, the number of times each
sequence was recovered is indicated in parentheses. The
dissociation constant for MBP as measured by yeast display is
also given. Unmutated residues originating from the mutagenesis
template are colored gray.
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