Figure 1.
FIGURE 1. SCAF8-CID binding to different phosphorylated
forms of the CTD measured by fluorescence anisotropy competition
titration experiments. Data points of the reference measurement
of SCAF8-CID titrated to 2 µM FAM-Ser(P)-2-CTD peptide are
presented as filled circles ( ). Data points for
competition experiments where 100 µM concentrations of
different non-fluorescently labeled CTD peptides competed
against 2 µM FAM-Ser(P)-2-CTD peptide are given as open
symbols. Fluorescence anisotropy data using
Ser(P)-2/Ser(P)-5-CTD peptide are presented as open diamonds (
)
(K[d] = 19 (+/- 2/2) µM), Ser(P)-2-CTD peptide as open
circles ( ) (K[d] = 68 (+/- 8/6)
µM), Ser(P)-2/Ser(P)-7-CTD as open squares ( ) (K[d] =
90 (+/- 4/3) µM), and Ser(P)-5-CTD as inverted open
triangles ( ) (K[d] = 330 (+/-
50/30) µM). The lines represent the best fit to data of
individual measurements.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2008,
283,
22659-22669)
copyright 2008.
). Data points for
competition experiments where 100 µM concentrations of
different non-fluorescently labeled CTD peptides competed
against 2 µM FAM-Ser(P)-2-CTD peptide are given as open
symbols. Fluorescence anisotropy data using
Ser(P)-2/Ser(P)-5-CTD peptide are presented as open diamonds (
)
(K[d] = 19 (+/- 2/2) µM), Ser(P)-2-CTD peptide as open
circles ( 
) (K[d] =
90 (+/- 4/3) µM), and Ser(P)-5-CTD as inverted open
triangles ( 
) (K[d] = 330 (+/-
50/30) µM). The lines represent the best fit to data of
individual measurements.