Figure 1 - full size

Figure 1. FIGURE 1. Construction of E. coli tRNA^Leu(UUR) bearing wobble modifications. A, chemical structures of the modified uridines introduced into the wobble position of E. coli tRNA^Leu(UUR): cmnm^5U and m^5U. B, cloverleaf structure of E. coli tRNA^Leu(UUR). U^*, the modified uridine. C, schematic depiction of the molecular surgery procedure used to construct E. coli tRNA^Leu(UUR) bearing the wobble modifications. The details are described under "Experimental Procedures." BAP, bacterial alkaline phosphatase; PNK, polynucleotide kinase.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2008, 283, 18801-18811) copyright 2008.