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Figure 1.
Fig. 1. (A) Two views of the ScGal80p^S0-ScGal4AD-NAD dimer.
The bottom view is rotated 90° around the horizontal axis
compared to the top view. ScGal80p is depicted as gray ribbons,
and the Gal4p-AD peptide and the NAD are shown in stick form
(Gal4p-AD peptide: purple; NAD atoms: yellow, carbon; red,
oxygen; blue, nitrogen; and green, phosphorus). Disordered
regions are shown as a dashed coil. The β-sheet regions of the
C-terminal domains form an extensive dimeric interface. (B) NAD
binding. Hydrogen-bonding interactions involving the NAD
dinucleotide are shown as dashed lines with the corresponding
distances indicated (Å). The side chain of W31 stacks on
the NAD nicotinamide ring. Atoms are color-coded as in (A) with
Gal80p carbons in gray, NAD carbons in yellow, and the Gal4p-AD
peptide carbons in purple (7).
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