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Figure 1.
Figure 1. Titration calorimetry of the interaction between
the HyHEL-10 Fv fragment and HEL. (A, B) (Top) Typical
calorimetric titration of hHyHEL-10 mutant Fv fragment (5
µM) with 50 µM HEL at pH 7.2 and 30°C; (bottom)
integration plot of the data calculated from the raw data. The
solid line corresponds to the best-fit curve obtained by
least-squares deconvolution. (A) hHyHEL-10; (B) HQ39KW47Y mutant.
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