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Figure 1.
FIGURE 1. Structure of the Spt16 NTD. Top, the residues in
each structural domain of Spt16-Pob3 are indicated (16, 23). 31
Spt16 homologs chosen to include the full spectrum of eukaryotes
were aligned and found to be about 40% identical to the yeast
sequence overall. The percent identity to the S. cerevisiae
sequence varied for each domain as indicated. All homologs
included the NTD. The C-terminal domain is broken into two
regions for this calculation, a less conserved but 50% acidic
region (960-1008) and a more highly conserved and neutral region
(1009-1029). D indicates the dimerization interface. Middle,
ribbon diagrams of the Spt16 NTD structure. The N-terminal lobe
is shown in turquoise; the C-terminal lobe containing the
putative binding cleft in blue, and the loop that encloses the
cleft in orange. Labels indicate the secondary structure
features. Bottom, secondary structures within the Spt16 NTD are
aligned with the sequence. Residues that are conserved among
over 70% of the 31 Spt16 homologs compared are highlighted in
red. Bars below the text indicate regions targeted for
site-directed mutagenesis, coded according to the severity of
the synthetic defect with pob3-Q308K (Table 2).
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