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Figure 1.
Figure 1. Crystal structure of bsmtnX: (A) A simplified
schematic of the methionine salvage pathway in B. subtilis
according to the schema proposed by Sekowska et al.[2] (B)
Stereo ribbon diagram of the bsmtnX monomer color-coded from
N-terminus (blue) to C-terminus (red). Helices (H1-H12) and -strands
1-
7)
are labeled. (C) Diagram showing the secondary structural
elements in bsmtnX superimposed on its primary sequence. The
helices, -strands,
-turns,
and -turns
are indicated. The -hairpin
is depicted as a red loop. (D) Grasp figure showing distribution
of surface electrostatic potential (red-negative (-6kT),
blue-positive (+6kT), and white-neutral, where k is the
Boltzmann constant and T is the temperature) for bsmtnX
positioned in an orientation similar to that in (B). The bound
Mg^2+ ion is shown as a green sphere and the position of the
buried Zn^2+ ion is indicated by a shaded sphere.
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