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Figure 1.
Fig. 1. Ferric citrate-mediated conformational changes in
FecA. (A) Superposition of FecA without (yellow) and with (blue)
ferric citrate (magenta). The front of the barrel has been
removed for clarity. The signaling domain is attached to the
plug by a flexible linker (dashed line). Binding ferric citrate
causes conformational changes in the barrel and the translation
of several apical loops of the plug toward the ferric citrate
molecule. (B) Close-up of the extracellular pocket as seen from
the solvent. Ferric citrate binding causes conformational
changes in L7 and L8 and the closing of the extracellular
pocket. (C) Close-up of the periplasmic pocket as seen from the
periplasm. Binding ferric citrate induces the unwinding of the
switch helix and changes in the relative position of the
TonB-box. All figures were prepared with PyMOL (41).
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