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Figure 1.
Figure 1. Schematic Diagram of the c-Abl Constructs Used and
the Structure of the Cap Region
(A) Abl^N-cap was used for
both the crystal structure and SAXS analyses. Residues from the
N-terminal cap in Abl^N-cap that were deleted are indicated with
gray shading, and residues that were included are highlighted in
pink.
(B) Surface representation of Abl^N-cap with the cap
region shown as a backbone model in pink. Residues that connect
the cap to the myristoyl but could not be modeled are shown as
pink spheres. Helix αI of the kinase domain is colored purple.
A black box indicates the region magnified in (C).
(C)
Hydrophobic surface rendition of Abl^N-cap showing cap
interactions with the SH2 domain and SH3-SH2 connector.
Increasing hydrophobicity of the surface is indicated with
darker shades of green. The cap is shown as sticks, where
carbon, nitrogen, and oxygen atoms are colored orange, blue, and
red, respectively. Labeled are residues that are well ordered
and make direct interactions with the protein. The water
molecule hydrogen bonded to Lys70 is shown as a blue sphere.
Molecular figures were generated with PyMOL (DeLano, 2002).
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