|
Figure 1.
FIGURE 1. Schematic representation of the disaccharide tri-
and tetrapeptide fragments resulting from digestion of P. putida
peptidoglycan with lysozyme. The calculated and experimentally
measured monoisotopic masses of the most abundant species are
indicated. Stereochemical information was taken from Refs. 8 and
15, and the glycosidic linkage was placed between C-1 of GlcNAc
and C-4 of MurNAc and not between C-1 of MurNAc and C-4 of
GlcNAc. This choice was based on the known preference of
lysozyme for the glycosidic link that connects C-1 of MurNAc to
C-4 of GlcNAc (16). S and P stand for substrate and product of
the enzymatic reaction.
|
