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Figure 1.
FIG. 1. A, the structure of P450cam highlighting the I
helix and the catalytically important residues, Thr252 and
Asp251. B, the P450 oxygen activation mechanism. Electron and
proton transfer to the ferrous dioxygen complex gives the
Fe(III)-OOH hydroperoxy intermediate. A second protonation of
the distal oxygen atom leads to heterolysis of the dioxygen O-O
bond and formation of Fe(IV)=O, the active hydroxylating species.
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