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Figure 1.
Figure 1. Photo-cross-links from the branch site and from the 3'
exon. (a) Polyacrylamide gel for visualization and isolation
of cross-linked [32P]D56 and [32P]D56(+1) species (bands A -C).
Lanes 1 and 2, unmodified D56; lanes 3 and 4, D56 containing a
s6dG at the branch site (A880); lanes 5 and 6, D56 ligated with
s6dG at the 3' end (D56(+1)). Cross-linking efficiencies are
3.3%, 5.1% and 1.2%, respectively, for bands A -C. (b) Mapping
of cross-links A and C by reverse transcriptase primer extension
(lanes 4 and 5, respectively; lane 3, primer extension control
on un-cross-linked material).
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