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Figure 1.
Figure 1. The mutase enzyme function and structure. (a) The
chemical reaction catalysed by the mutase enzyme. The co-factor
FAD and FADH - are shown. Ring positions numbers are referred to
in the text. (b) The bicyclic mechanism, which does not require
direct involvement of the FAD.8 (c) The redox mechanism, in
which one electron is transferred to the substrate.11 The
electron is transferred from FADH - to create a radical that
then re-arranges to give product. (d) The covalent intermediate
mechanism, in this N5 attacks C1 in a nucleophilic manner. The
covalent intermediate then re-arranges to give product. The
presence of the covalent intermediate was detected by mass
specotrometry.12
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