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Figure 1.
Fig. 1. Biochemistry of the reaction and the amino acid
sequence and three-dimensional structure of LCF D3. (A)
Bioluminescent reaction of dinoflagellate luciferin, a
chlorophyll-like open tetrapyrrole. The arrow shows the position
of enzymatic oxidation. (B) The sequence of LCF D3 with regions
of  -helix ( ) and
 -strand ( )
annotated. The Gly-rich sequence (italicized and underlined)
connects the N-terminal subdomain to the -barrel comprising
strands 5to 14. The four N-terminal
histidines (899, 909, 924, and 930) are shown in red and
italicized. Two underlined six-residue sequences show the
beginning and end of the highly conserved region (991-1136)
found in LCFs of all seven species examined (23). (C) A ribbon
diagram of the crystal structure of D3 with helices and -strands
numbered and color coded as in B. The two N-terminal helices
(light blue) and the helix-loop-helix motif (green) are at the
top, and the -barrel (dark blue) is
below. Ribbon diagrams were produced by using MOLSCRIPT (42) and
RASTER3D (43).
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