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Figure 1.
Fig. 1. Structures of hTK1 and Uu-TK. (A) Structural
alignment of the sequences of TKs from human (P04183 [GenBank]
), Dictyostelium discoideum (AAB03673 [GenBank]
1), Vaccinia virus (AAB96503 [GenBank]
1), B. cereus (ZP_00241105.1), E. coli (NP_287483 [GenBank]
.1), and U. urealyticum (U. parvum) (NP_078433 [GenBank]
). Secondary structure elements for hTK1 are shown above the
alignment in brown, and those for Uu-TK are shown below in
green. The P loop and the two zinc coordinating sequences are
boxed. (B) Subunit structure of hTK1 with dTTP colored according
to atom type. Mg2+ is shown in yellow, and Zn2+ is shown in
gray. (C) Subunit structure of Uu-TK with dTTP, Mg2+, and Zn2+
shown in same colors as in B. hTK1 and Uu-TK are tetramers. (D
and E) hTK1 (D) and Uu-TK (E) tetramers shown in different
views. The C-terminal helix in Uu-TK interacts with a helix on
the adjacent monomer.
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