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Figure 1.
Figure 1. The structure of homotetrameric MDH from C. aurantiacus is shown in a ribbon diagram in the upper panel.
The tetramer consists of two dimers (AB and CD). The NAD cofactor bound to the catalytic centre is shown in yellow.
The rectangle indicates the location of the electrostatic network around Glu165 at the dimer--dimer interface in CaMDH
(note that the network occurs four times in the tetramer). The lower panel shows a stereo view of the environment of
Glu165 in subunit A, with backbones coloured according to the scheme used in the upper panel. The side-chains are
shown in ball and stick in atom colours, Cd
2C
bound to Glu165 is shown in yellow. Dotted lines indicate interacting
atoms, located within 3 Å of each other.
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