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Figure 1.
Figure 1 Overview of poliovirus 3D^pol RdRp structure. (A)
Comparison of the original partial structure (yellow) with the
complete structure shown with the fingers domain in red, the
palm in gray, the thumb in blue, and the active site colored
magenta. The N-terminal strand (residues 12 -36) of the original
structure that descended toward the active site is shown in
green. The two structures were superimposed using the backbone
atoms of the active site GDD motif and three residues on either
side of it (i.e. residues 324 -332). (B) Superimposition of the
thumb domains from the original structure (yellow) and new
complete structure (blue) showing that the thumb structure is
largely unchanged by the two mutations (L446D and R455D) used to
break Interface I and crystallize 3D^pol in a new lattice. The
side chains of Phe30 and Phe34 are shown in green for the
original structure and red for the new complete structure. (C)
Top view of the complete 3D^pol structure highlighting the
individual fingers of the fingers domain. The index finger is
shown in green, the middle finger in orange, the ring finger in
yellow, and the pinky finger in pink. As in (A), the palm is
shown in gray, the thumb is in blue, and the active site is
colored magenta. Phe30 and Phe34 are shown as sticks, Pro119 on
the pinky finger is indicated with spheres, and glycines 117 and
124 are colored in cyan. (D) Bar representation of the 3D^pol
sequence colored according to the structural elements shown in
(C). Sections of the sequence in the palm are in gray and the
numbers correspond to the first residue in a given structural
motif.
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