Figure 1.
Figure 1. FkpA prevents MalE31 aggregation. Cells
expressing chromosomally encoded MalE-wt or MalE31, transformed
by pTrc99 or pTfkp, were grown in rich medium at 37°C for 3 h,
and then fractionated from spheroplasts. Periplasmic (soluble)
and membrane (insoluble) fractions, corresponding to 5 x 10^9
cells, were analyzed on SDS-polyacrylamide (12.5%) gel stained
by Coomassie blue. (Lane 1), Strain PD28 ( malE)
carrying pTrc99. (Lane 2) Strain MC4100 (malE) carrying pTrc99.
(Lane 3) Strain JMB5 (malE31) carrying pTrc99. (Lane 4) JMB5
cells carrying pTfkp.
The above figure is reprinted
by permission from the Protein Society:
Protein Sci
(2003,
12,
577-585)
copyright 2003.
malE)
carrying pTrc99. (Lane 2) Strain MC4100 (malE) carrying pTrc99.
(Lane 3) Strain JMB5 (malE31) carrying pTrc99. (Lane 4) JMB5
cells carrying pTfkp.