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Figure 1.
Fig. 1. Ribbon models of the TEV (C151A) and TEV(S219D)
protease structures. A, stereo diagram of the S219D monomer
(molecule A). The residues that compose the catalytic triad and
the N-terminal His tag are depicted as ball-and-stick models
(carbon, violet; nitrogen, blue; oxygen, red; and sulfur,
yellow). The peptide product is also colored blue to distinguish
it from the protein. B, the C151A dimer. C, the S219D dimer.
Molecule B (red) is shown in the same orientation in B and C to
illustrate the difference between the two dimers. The peptides
are colored blue. Residue 151, which is Ala in the C151A
protease and Cys in the S219D protease, is depicted as a
ball-and-stick model. Residues 230-236, which are visible only
in molecule A of the S219D protease, are colored yellow.
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