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Figure 1.
Figure 1. Comparison of the Structures of the g and a
Appendages(A) Superposition of the Ca traces of g appendage
(purple) and a appendage N-terminal subdomain (green). The two
overlay with an rms deviation of 1.8 Å for 96 Ca atoms.(B)
Schematic representation of the g appendage domain. Point
mutations that abrogate binding to g-synergin and Eps15 are
shown in yellow in ball and stick representation. All protein
structure pictures were made using Aesop (M.E.M. Noble, personal
communication).(C) Schematic representation of the a appendage
domain. The N-terminal subdomain is in green and the C-terminal
subdomain in gold.(D) Structure-based sequence alignment of g
appendages from human, mouse, Arabidopsis, and smutfungus, mouse
g2-appendage, human GGA1 appendage, and the N-terminal subdomain
of the a appendage. The positions of b strands are marked by
arrows and a helices with rods (purple human g appendage and
green a appendage). Conserved residues are indicated by gray
shading and residues identical between the g and a appendages
are marked with an asterisk.(E) Ca trace of the g appendage in
stereo representation.
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