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Figure 1.
Fig. 1. Apical domain constructs of GroEL. A, GroEL
apical domain constructs indicating the amino acid residues
included and modifications made in the proteins expressed for
these studies. B, ribbon diagram showing a comparison of the
overall structures of five independent GroEL apical domains:
C-His ApEL (determined here) ( purple), N-His ApEL (22) (red),
ApEL-(191-336)·peptide complex (19) (yellow), intact WT
GroEL (9) (blue), and a GroEL·ADP complex (45) (green).
The diagram shows the conformational flexibility in the region
around helices H and I.
C, r.m.s. deviation plot showing the differences in C-  positions
between C-His ApEL and N-His ApEL (22) (unbroken line), and
C-His ApEL and an ApEL-(191-336)·peptide complex (19)
(dotted line).
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