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Figure 1.
Fig. 1. Orientation of sugars bound in the MBP site. In A
and B, the structure of the terminal mannose of the Man  (1,2)
branch of Man[6]GlcNAc[2]Asn (13) defining orientation I is
shown on the left, and the structure of MeMan bound
to MBP-C (14) defining orientation II is shown on the right.
Ca^2+ coordination bonds are shown as long dashed lines,
hydrogen bonds are shown as short dashed lines, and van der
Waals contacts are shown as dotted lines. A, a view of the MBP
binding site roughly perpendicular to the face of the pyranose
ring. B, a view of the site rotated ~90° around the vertical
axis with respect to A. For clarity, MBP-A residues His189 and
Ile^207 and MBP-C residues Val194 and Val212 are shown only in
B. The conformation of MBP-A His189 is determined by a hydrogen
bond between His189 N[  1] and
the backbone NH of Gly191 (not shown).
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