Figure 1 - full size

 

Figure 1.
Fig. 1. The argininosuccinate synthetase mechanism. Step 1, activated citrulline-adenylate is formed, releasing inorganic pyrophosphate. Step 2, nucleophilic attack by aspartate amino group forms argininosuccinate and releases AMP.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2002, 277, 13074-13081) copyright 2002.