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Figure 1.
Figure 1: Mammalian SRP and Alu domain RNA constructs. a,
Mammalian SRP with SRP RNA bound to six proteins. The Alu domain
is coloured red (SRP9), green (SRP14), blue (Alu RNA 5' domain)
and cyan (Alu RNA 3' domain). b, SA86, the minimal Alu RNA
folding domain. S-domain RNA is replaced by a GUAA tetraloop
(grey). The green arrow indicates flexibility between the RNA 5'
and 3' domains. Hydroxyl-radical footprints of SRP9/14 on SRP
RNA are highlighted in magenta^21. c, SA88, a circular
permutation of SA86. The original 5' and 3' ends are connected
by a mono-uridine linker (black), constraining the RNA 5' and 3'
domains to stack in an extended conformation (red bar). The GUAA
tetraloop is replaced by a terminal stem (grey). The asymmetric
internal loop is highlighted (yellow).
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