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Title
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Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli.
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Authors
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E.J.Hebert,
G.R.Grimsley,
R.W.Hartley,
G.Horn,
D.Schell,
S.Garcia,
V.Both,
J.Sevcik,
C.N.Pace.
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Ref.
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Protein Expr Purif, 1997,
11,
162-168.
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PubMed id
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Abstract
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The genes for three small ribonucleases from different strains of Streptomyces
aureofaciens have been cloned and expressed in Escherichia coli. The
purification of these ribonucleases from the periplasmic space is described. The
yields range from 10 to 50 mg of protein per liter of culture medium. The molar
absorption coefficients, isoelectric pH values, and pH of optimum activity are
reported.
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