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Title
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Preparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
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Authors
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A.Lapko,
A.Müller,
O.Heese,
K.Ruckpaul,
U.Heinemann.
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Ref.
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Proteins, 1997,
28,
289-292.
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PubMed id
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Abstract
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Bovine adrenodoxin was cross-linked to adrenodoxin reductase with
1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide. Mass spectrometry showed the
reaction product to be a 1:1 complex of the two proteins with M(r) = 64,790 +/-
50. The cross-linked complex showed cytochrome c reductase activity and could be
crystallized by hanging-drop vapor diffusion. Crystals of the
adrenodoxin-adrenodoxin reductase complex are hexagonal, space group P6(1)22 or
P6(5)22, with a = 93.26 A and c = 612.20 A and diffract to 2.9 A resolution at
100 K. Assuming two cross-linked complexes per asymmetric unit yields a
reasonable V(M) of 2.97 A3/Da.
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